Lipoprotein Analysis Using Agarose Gel Electrophoresis and Differential Staining of Lipids
نویسندگان
چکیده
منابع مشابه
Quantitative measurement of lipoprotein surface charge by agarose gel electrophoresis.
The electrophoretic mobilities of low density lipoprotein (LDL) and six pure proteins in a 0.5% agarose gel have been compared to literature electrophoretic mobility values determined by the Tiselius moving boundary method. There is a strong correlation (r = 0.99) between the electrophoretic mobilities determined by the two techniques. The electrophoretic behavior of charged particles smaller t...
متن کاملAgarose gel electrophoresis.
MATERIALS 6x Gel-loading buffer Agarose solutions (please see Step 3) DNA samples DNA size standards Samples of DNAs of known size are typically generated by restriction enzyme digestion of a plasmid or bacteriophage DNA of known sequence. Alternatively, they are produced by ligating a monomer DNA fragment of known size into a ladder of polymeric forms. DNA staining solution For a discussion of...
متن کاملAgarose gel electrophoresis.
Agarose gel electrophoresis is used to separate DNA or RNA molecules based upon their size.
متن کاملAlkaline agarose gel electrophoresis.
INTRODUCTION Alkaline agarose gels are run at a pH that is sufficiently high to denature double-stranded DNA. The denatured DNA is maintained in a single-stranded state and migrates through the alkaline gel as a function of its size. Alkaline agarose gels are used chiefly to measure the size of first and second strands of cDNA (Construction of cDNA Libraries Stage 1: Synthesis of First-strand c...
متن کاملResolving Transferrin Isoforms via Agarose Gel Electrophoresis.
OBJECTIVE Cerebrospinal fluid (CSF) leaks may present as rhinorrhea or otorrhea and can lead to life-threatening complications if not detected. The usefulness of the morpholinopropanesulfonic acid (MOPS)-histidine buffer in detecting β₂-transferrin, which is only found in the cerebrospinal fluid, was compared with the standard barbital buffer. METHODS We evaluated 20 aural or nasal patient sp...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Atherosclerosis and Thrombosis
سال: 2001
ISSN: 1340-3478,1880-3873
DOI: 10.5551/jat1994.8.7